A study of stem cell marker lineage marker gene expression and clonal expansion in TGFβR2iSMC-Apoe mice Chen et al. Related to Fig 4

Characterization of TGFβR2iSMC-Apoe-/- mice after 1 month of high cholesterol high fat by histology, imaging mass cytometry (IMC), histoCAT, CyTOF, and immunocytochemistry. Aortic smooth muscle cells from TGFβR2iSMC-Apoe-/- express stem cell markers (CD105, CD73, CD90, Sca-1, CD44), mesenchymal cell markers (Osteopontin, Aggrecan, Adiponectin). To show clonal origin of smooth muscle cell-derived mesenchymal cells in TGFβR2iSMC-Apoe mice-/-, we replaced mTmG reporter strain TGFβR2iSMC-Apoe mice generating Myh11CreERT2;Tgfbr2f/f;Apoe-/-;Confettif/f and Myh11CreERT2;Apoe-/-:Confettif/f (control) lines.

对TGFβR2iSMC-Apoe-/-小鼠在一个月高胆固醇高脂肪饮食后通过组织学、成像质谱流式细胞术（IMC）、组织切片分析（histoCAT）、细胞质流式细胞术（CyTOF）和免疫细胞化学进行表征。TGFβR2iSMC-Apoe-/-小鼠的主动脉平滑肌细胞表达干细胞标志物（CD105、CD73、CD90、Sca-1、CD44）和间质细胞标志物（骨桥蛋白、聚集素、脂联素）。为了展示TGFβR2iSMC-Apoe-/-小鼠中平滑肌细胞来源的间质细胞的克隆起源，我们替换了mTmG报告菌株TGFβR2iSMC-Apoe小鼠，生成Myh11CreERT2;Tgfbr2f/f;Apoe-/-;Confettif/f（实验组）和Myh11CreERT2;Apoe-/-:Confettif/f（对照组）两条线。