Single-nucleotide resolution m6A peak and their unqiue characteristics in developing mouse forebrain

We investigated the accurate location and methylation stochiometry for Mettl14-dependent m6A peaks in the samples from P0 mouse forebrains using m6A-SAC-seq. We found that m6A-modified transcripts showed strong enrichment for genes associated with synapse organization, axonogenesis, positive regulation of cell projection organization, and regulation of synapse, implying that m6A-modified RNAs play a key role in neuronal differentiation during early postnatal cortical development . Overall design: To investigate the single-nucleotide resolution of m6A peak and their quantitative stoichiometry in P0 forebrain, we conducted m6A-SAC-seq from WT and Nestin-Cre Mettl14 conditional knockout mouse. By comparing WT and Mettl14 cKO, we extracted Mettl14 dependent m6A peak in P0 forebrain.