Single-cell RNA-seq of NMNAT1 knockout (NMNAT1KO) human induced pluripotent stem cell (hiPSC)-derived retinal organoid. Homo sapiens

Nicotinamide mononucleotide adenylyltransferase 1 (NMNAT1) is one of causative genes of Leber congenital amaurosis (LCA), an inherited retinal degeneration that appears at birth. We previously revealed that Nmnat1 has essential roles for retinal progenitor cell survival during mouse retinal development. To understand roles of NMNAT1 during human retinal development, NMNAT1KO hiPSC was established and used for induction of retinal organoid. Here we performed single-cell RNA-seq to examine what kind of changes in cell type composition occurred in normal and NMNAT1KO groups during retinal organoid development.