mRNA expression profiling A549 lung cancer cells following PPARGC1A suppression

PPARGC1A oppositely regulates cancer metastasis in melanoma, breast, and pancreatic cancer; however, little is known about its impact on lung cancer metastasis. We generated gene-expression profile of control and PPARGC1A suppressed A549 cells, a lung adenocarcinoma cell line that expresses moderate levels of PPARGC1A to investigate the role of this gene in lung cancer metastasis. Overall design: Lentivirus expressing shRNA against PPARGC1A or control was infected into A549 lung cancer cells. The infected cells were selected with puromycin for 5 days, and then total RNA was used to analyze gene expression profiling.