造纸污泥成分分析数据集
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①为了进行污泥有机质分析，将0.05 g的污泥样品放在50 mL的PTFT消化管中，然后加入20 mL 0.136 mol/L的K2Cr2O7-H2SO4消化液。消化管置于加热至180 ℃的石墨分离器中反应5 min后冷却。取1 mL消化后的溶液，加入一个小管，放在顶空瓶中，然后将1 mol/L 5 mL的草酸溶液慢慢加入顶空瓶，密封和摇匀，使消化液和草酸溶液均匀混合。然后，将顶空瓶放置在顶空自动采样器中进行自动HS-GC测量。在本实验中，样品在60 ℃的顶空烘箱中剧烈摇晃3 min、平衡10 min后自动GC进样分析。其它顶空操作条件为：定量环温度70 ℃、传输线温度 80 ℃；增压和载气压力分别为2.00和1.5 bar；增压、定量环平衡和样品-定量环充满时间分别为0.2 min、0.05 min和0.2 min。气相色谱柱和TCD检测器的工作温度分别为105 ℃和250 ℃。氮气作为气相色谱载气（流速为3.8 mL/min）。通过酸化相同质量的样品（0.005 mol/L盐酸溶液，4 mL）测定碳酸盐的干扰。②将好氧活性污泥用生理盐水冲洗3-4次，然后充气。同时，采用0.45 μm滤膜过滤废水，曝气1 h，使溶解氧（DO）浓度低于8.0 mg/L。将15 mL混合样品（即5 mL污泥和10 mL曝气废水）置于21.6 mL的样品瓶中，并置于培养箱内，在20 °C下以150转/分的速度搅拌。经过不同培养时间后，从培养箱中取出小瓶，每个小瓶中注入0.5 mL 0.005 mol/L的盐酸，结束样品瓶中微生物的代谢。然后，将样品瓶转移到顶空自动进样器中，测量氧气和二氧化碳的TCD峰面积。每个样品重复分析5次。
① To analyze the organic matter in sludge, 0.05 g of sludge sample was transferred into a 50 mL PTFT digestion tube, followed by addition of 20 mL of 0.136 mol/L K₂Cr₂O₇-H₂SO₄ digestion solution. The digestion tube was placed in a graphite separator heated to 180 ℃ for 5 min, then cooled. Next, 1 mL of the digested solution was transferred into a small tube, which was then placed into a headspace vial. Subsequently, 5 mL of 1 mol/L oxalic acid solution was slowly added into the headspace vial, and the vial was sealed and shaken thoroughly to achieve uniform mixing of the digested solution and oxalic acid solution. The headspace vial was then placed into a headspace autosampler for automated HS-GC measurement. In this experiment, the sample was vigorously shaken in the headspace oven at 60 ℃ for 3 min and equilibrated for 10 min, followed by automated GC injection analysis. Other headspace operating parameters are as follows: sample loop temperature 70 ℃, transfer line temperature 80 ℃; pressurization and carrier gas pressures were 2.00 bar and 1.5 bar, respectively; the durations for pressurization, sample loop equilibration, and sample loop filling were 0.2 min, 0.05 min, and 0.2 min, respectively. The operating temperatures of the gas chromatography column and TCD detector were 105 ℃ and 250 ℃, respectively. Nitrogen was used as the GC carrier gas with a flow rate of 3.8 mL/min. The interference from carbonates was determined by acidifying equivalent-mass samples with 4 mL of 0.005 mol/L hydrochloric acid solution. ② The aerobic activated sludge was rinsed with normal saline for 3-4 times, then aerated. Meanwhile, the wastewater was filtered through a 0.45 μm microporous membrane, and the filtrate was aerated for 1 h to reduce the dissolved oxygen (DO) concentration to below 8.0 mg/L. A 15 mL mixed sample (i.e., 5 mL sludge and 10 mL aerated wastewater) was transferred into a 21.6 mL sample vial, which was then placed in an incubator and agitated at 150 rpm and 20 ℃. After different incubation periods, the vials were withdrawn from the incubator, and 0.5 mL of 0.005 mol/L hydrochloric acid was injected into each vial to terminate the microbial metabolism in the sample vial. Then, the sample vials were transferred to a headspace autosampler to measure the TCD peak areas of oxygen and carbon dioxide. Each sample was analyzed in 5 replicates.
