遇见数据集

OME-Zarr 3D hiPSCs with 3D labels & 3D measurements, 2x2 field of views

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Zenodo2024-08-12 更新2026-05-26 收录
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These are 2 small OME-Zarr files of the data from 10.5281/zenodo.7057076. The images have been processed using Fractal, with this workflow. It ran with fractal-server==1.0.8, fractal-client==1.0.5 and fractal-tasks-core==0.7.4. Both Zarr files are Zip-compressed to allow easier upload & download from Zenodo. 20200812-CardiomyocyteDifferentiation14-Cycle1.zarr contains 3 3D channels, a nuclear segmentation produced by cellpose as labels and 2 tables: A region of interests table for the 4 field of views, as well as measurements performed with napari-skimage-regionprops. 20200812-CardiomyocyteDifferentiation14-Cycle1_mip.zarr contains the same 3 channels, but as maximum intensity projections. It contains nuclear segmentation through cellpose. It also contains 2 tables: The region of interests like in the 3D data, as well as measurements performed with napari-skimage-regionprops. The tables are stored in the OME-Zarr file according to the proposed OME-NGFF table spec in AnnData. The 3 channels are: - 0: DAPI, nuclear stain - 1: nanog, antibody staining with Bio-Techne AG, AF1997-SP, Lot KKJ0617121 for the stemness marker nanog - 2: Lamin B1, antibody staining with Abcam, ab16048, Lot GR3244890-2 for the nuclear envelope marker Lamin B1 This OME-Zarr file is an extended version of https://zenodo.org/record/7120354, now with 3D segmentation and measurements for 3D objects.

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Zenodo
创建时间:
2023-02-24
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