Effect of overexpression A20 on gene expression during of TM3 Leydig cells. Effect of overexpression A20 on gene expression during of TM3 Leydig cells

To investigate the impact of A20 on testosterone synthesis in the context of age-related inflammation, we employed lentiviral constructs to establish TNFAIP3-overexpressing TM3 cell lines. Subsequently, we conducted gene expression profiling analysis using RNA-seq data obtained from both normal TM3 cells and TNFAIP3-overexpressing TM3 cells. Overall design: Comparative gene expression profling analysis of RNA-seg data for TM3 cells and its overexpression derivative (O/E A20)