five

Supplementary tables for: Genetic drift does not outweigh natural selection at Toll-like receptor genes in the populations of Galápagos mockingbirds

收藏
Figshare2022-10-25 更新2026-04-08 收录
下载链接:
https://figshare.com/articles/dataset/Supplementary_tables_for_Natural_selection_outweighs_genetic_drift_at_Toll-like_receptor_TLR_genes_in_the_populations_of_Gal_pagos_mockingbirds/12180027/3
下载链接
链接失效反馈
官方服务:
资源简介:
The files contain supporting data for a manuscript about Toll-like receptor diversity in Galapagos mockingbirds. In this project we sequenced three Toll-like receptors (TLR1B, TLR4 and TLR15) by pair-end Illumina amplicon sequencing and the files presented here contain information about the sequence quality, genotyping and refined genotypes of individual mockingbirds. More details about methods can be found in the manuscript that will be published soon.<br>Description of the files:Supporting information 2 - amplicon statistics.tsv<br>This file contains various statistics of sequencing data presented per each amplicon (PCR_ID). The columns contain: number of raw sequencing reads (Raw_read_pairs), number of read pairs that survived trimmomatic filtering (both_survived), percentage of those reads from total (bs_perc), number of forward only reads that survived trimmomatic filtering (F_survived), their percentage (Fs_perc), number of reverse only reads that survived trimmomatic filtering (R_survived), their percentage (Rs_perc), number of reads that did not pass quality filtering (dropped), percentage of dropped reads( drop_perc), average sequence length (avg_sequence_length) and its range (Length_range), total number of refined sequences (Total Sequences), GC content (%GC).<br>Supporting information 3 - amplicon frequency.tsvThe file presents number of unique haplotypes per each TLR amplicon (AMPLICON_ID). The frequency of the five most frequent haplotypes shown (Frequency_{1..5}). If the ratio of frequencies of second to first haplotype (ratio_second_first) was higher then 1/3 we considered the locus heterozygous. <br>Supporting information 4 - genebank accession numbers.tsvThe file contains link between accession number in Genebank database and haplotype name (Allele name) used in genotype table (next file). <br>Supporting information 5 - individuals and their genotypes.tsvThis file contains TLR genotypes for each individual (sample name) belonging to particular island (population). Information about genotype (TLR1Ballele-1, TLR1Ballele-2, etc.) together with haplotype sequences stored in Genebank data can be used for recovery of the population level dataset.<br><br>Supporting information 6 multiqc_report.html<br>Multiqc report of amplicon dataset after trimmomatic filtering.
提供机构:
Vinkler, Michal; Štefka, Jan; Vlcek, Jakub; Miláček, Matěj
创建时间:
2022-05-23
二维码
社区交流群
二维码
科研交流群
商业服务