Impact of MEM40 and IFNB on human DC activation markers

To determine the impact of MEM40 and IFNB on normal human donor monocyte-derived DCs (Mo-DCs) phenotype, we used an experimental design where human A549 lung cancer cells were first infected with Ad-Null or adenoviruses expressing human IFNB, MEM40 or MEM40 + IFNB, followed by removal of virus-containing supernatant and co-culture with Mo-DCs. We used the above design followed by sorting of MHC-II+ CD11c+ cells for bulk RNA-sequencing. Overall design: A549 were infected with ISF35 or/ and IFNB viruses followed by cocultured with human Mo-DCs (CD14+ PBMC were purified and stimulated by IL-4 and GM-CSF). 2 days later, all cells were collected for cell sorting. RNA-seq was used to determine changes in gene expression after sorting HLA-DR+CD11c+ DCs derived from monocytes of 3 healthy donors.