Paired single nucleus RNA and ATAC sequencing on the role of diet and aging in hematopoietic stem and progenitor cells

We performed joint single-nucleus RNA (snRNA-seq) and ATAC (snATAC-seq) sequencing on hematopoietic stem and progenitor cells (LSK) isolated from mouse bone marrow of mice of different ages and dietary treatments. Mice were either fed ad libitum continually until bone marrow isolation at young (yAL) or old (oAL) age, or underwent a mild dietary restriction (DR; 30% reduction in food intake by weight) for the two weeks prior to bone marrow isolation at young (yDR) or old (oDR) age to investigate early response to nutrient deprivation. Each of these four samples contained pooled cells from two mice. Profiles of single-nucleus transcriptomes and epigenomes, assayed using snRNA-seq and scATAC-seq, across sorted LSK cells from mouse bone marrow for mice of different diets and ages.