Effect of Transforming Growth Factor-beta 1 on the Rat Renal Mesangial Cell Transcriptome
收藏DataCite Commons2023-06-28 更新2025-04-17 收录
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https://melbourne.figshare.com/articles/dataset/Effect_of_Transforming_Growth_Factor-beta_1_on_the_Rat_Renal_Mesangial_Cell_Transcriptome/23591004/1
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资源简介：
Rat renal mesangial cells (SciencCell, #R4200) were grown to sub-confluence on poly-L-lysine coated flasks in mesgnaial cell medium (SciencCell, #R4201) supplemented with 2�S, growth supplement (SciencCell, #R4252) and pen/strep. Cells were quiesced for 48h and then treated for 24h with human recombinant TGF-beta 1 (5 ng/mL; Sigma#T7039) in complete media (n=4) or fresh media without growth factor (n=4). Total RNA was extraced from cell pellets using standard protocols. Whole RNA stranded libraries were prepared using Illumina's Ribo-zero Gold protocol and sequenced on an Illumina NovaSeq platform (150bp PE). After demultiplexing and quality control, the data was then processed through RNA-seq expression analysis workflow, which included trimming, alignment, transcript assembly, feature quantification and, differential expression analysis with edgeR (verison 3.38.4).
提供机构：
University of Melbourne
创建时间：
2023-06-28
