Lab-scale sequencing batch reactors of AS under antibiotic selection pressure Metagenome. Lab-scale sequencing batch reactors of AS under antibiotic selection pressure

We used metagenomic assembly and binning methods to investigate the effects of different antibiotics on the microbial community assembly, particularly for the comammox and other microorganisms involved in nitrification in lab-scale activated sludge systems. The concentrations of each antibiotic were step-wise increased from 20 mg/L to a final level. We retrieved metagenome-assembled genomes (MAGs) from the control group and AS-treated by kanamycin, ampicillin, lincomycin, and trimethoprim. The main objective of this study is to investigate how comammox and other nitrifiers respond to the addition of these antibiotics.