Nuclear receptor corepressor (NCOR) and HDAC3 in hypothalamus [ChIP-Seq]

We determined genomic binding of HDAC3 in mouse hypothalamus by ChIP-seq, and identified target genes of the NCOR/HDAC3 complex in hypothalamus of NS-DADm (mutated deacetylase activation domain in NCORs) mice by RNA-seq. Hypothalamus from 7-8 mice were pooled for one ChIP. Multiple independent ChIP samples and Input samples were pooled to generate 1 ChIP library and 1 input library followed by sequencing. For RNA-seq, total RNAs from hypothalamus of 3 WT mice and 3 NS-DADm mice were separately prepared to generate 6 libraries, followed by sequencing