植物整合紫外光与生长素信号的动态发育与蛋白质基础的研究数据

本数据集采集于厦门大学生命科学学院和福建农林大学海峡联合研究院，数据量约为300M。利用分子生物学和遗传学等方法探究紫外光对生长素信号应答基因调控的分子机制。通过分析核内UVR8调控的UV-B光应答的转录组变化，鉴定核内UVR8调控的激素应答基因，解析核内UVR8在生长素和茉莉酸等激素信号应答中的作用；通过筛选与核内UVR8共同调控基因表达的转录因子,鉴定到UV-B光和UVR8主要通过PIF4调控生长素应答基因表达，揭示UV-B光受体和转录因子介导光信号应答与生长素信号应答的分子机制；通过对拟南芥根尖的重力反应测试，发现UV-B光照导致根尖重力反应减弱。通过分析脂筏的流动性及激素的水平，发现UV-B光通过介导脂筏重排并刺激水杨酸升高来抑制植物根尖重力响应；利用rem1.2和rem1.2 1.3突变体，检测到水杨酸介导的根尖重力丧失依赖于Remorin介导的细胞膜脂筏调控途径，并调节了PIN2介导的生长素极性运输，水杨酸特异性刺激细胞膜上PIN2的区域化点状分布，抑制PIN2蛋白的内吞速度以及在细胞膜上的动态扩散，揭示了紫外光整合水杨酸和生长素信号调控植物根尖重力响应的分子机制；通过观察开花植物感受光信号的开闭规律，发现花瓣基部是开花生物钟的重要响应部位，花瓣的开花生物钟受到生长素的调控。通过分析花瓣基部的细胞形态、建立作物观测模型及分析开花闭合过程中差异表达基因，发现囊泡运输和细胞壁修饰与植物开花节律有相似趋势，揭示了生长素通过介导细胞壁修饰基因的节律调控来调控植物响应开花节律，预测了花开放-闭合过程中的生长素合成、信号和运输协调模型，并建立了作物动态运动的生理和细胞生物学观测体系；获得一种用于UV-B光下植物细胞FRAP实验的装置，方便观察UV-B光对植物细胞内分子动态的影响。

This dataset was collected from the School of Life Sciences of Xiamen University and the Joint Haixi Institute of Fujian Agriculture and Forestry University, with a total data volume of approximately 300 MB. Molecular biology and genetics approaches were employed to investigate the molecular mechanisms underlying the regulation of auxin signaling-responsive genes by ultraviolet (UV) light. By analyzing transcriptomic changes of UV-B light responses regulated by nuclear-localized UVR8, hormone-responsive genes regulated by nuclear UVR8 were identified, and the roles of nuclear UVR8 in the signaling responses of hormones including auxin and jasmonic acid were elucidated. By screening transcription factors that co-regulate gene expression with nuclear UVR8, it was determined that UV-B light and UVR8 primarily regulate auxin-responsive gene expression via PIF4, revealing the molecular mechanisms by which UV-B photoreceptors and transcription factors mediate crosstalk between light signaling responses and auxin signaling responses. Through gravitropic response assays on Arabidopsis thaliana root tips, it was found that UV-B light exposure attenuates the gravitropic response of root tips. By analyzing the fluidity of lipid rafts and the levels of hormones, it was discovered that UV-B light inhibits plant root tip gravitropic responses by mediating lipid raft rearrangement and stimulating the elevation of salicylic acid (SA). Using rem1.2 and rem1.2 1.3 mutants, it was detected that salicylic acid-mediated root tip gravitropic loss relies on the Remorin-mediated plasma membrane lipid raft regulatory pathway, and this process regulates PIN2-mediated polar auxin transport. Salicylic acid specifically stimulates the compartmentalized punctate distribution of PIN2 on the plasma membrane, inhibits the endocytic rate of PIN2 protein and its dynamic diffusion on the plasma membrane, thus revealing the molecular mechanism by which ultraviolet light integrates salicylic acid and auxin signaling to regulate plant root tip gravitropic responses. By observing the opening and closing patterns of flowering plants in response to light signals, it was found that the petal base is an important responsive site for the flowering circadian clock, and the flowering circadian clock of petals is regulated by auxin. By analyzing the cell morphology of petal bases, establishing crop observation models, and analyzing differentially expressed genes during flower opening and closing processes, it was revealed that vesicle transport and cell wall modification exhibit similar trends to plant flowering rhythms. This study uncovered that auxin regulates plant responses to flowering rhythms by mediating the circadian regulation of cell wall modification genes, predicted a coordinated model of auxin biosynthesis, signaling and transport during flower opening-closing cycles, and established physiological and cell biological observation systems for crop dynamic movements. A device for fluorescence recovery after photobleaching (FRAP) experiments on plant cells under UV-B light was developed, which facilitates the observation of the effects of UV-B light on molecular dynamics within plant cells.

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该数据集聚焦于植物中紫外光与生长素信号的整合调控机制研究，通过分子生物学和遗传学方法，揭示了紫外光如何通过UVR8受体、转录因子PIF4等影响生长素应答基因表达，并涉及根尖重力响应、水杨酸信号和开花节律的调控。数据来源于厦门大学和福建农林大学，包含约607.4MB的研究文件，支持对动态发育和蛋白质基础的深入分析。