Recombination data for the repeat-based holocentromere-harbouring genome of R. breviuscula

Centromeres strongly affect (epi)genomic architecture and meiotic recombination dynamics influencing the overall distribution and frequency of crossovers. Here, we studied how recombination is regulated and distributed in the holocentric plant Rhynchospora breviuscula, a species lacking localised centromeres. Combining immunocytochemistry, chromatin analysis, and high-throughput single-pollen sequencing, we discovered that crossover frequency is distally biased, in sharp contrast with the diffused distribution of hundreds of centromeric units and (epi)genomic features. Remarkably, we found that crossovers were abolished inside centromeric units but not in their proximity indicating the absence of a canonical centromere effect. We further show that telomere-led synapsis of homologs is the feature that best explains the observed recombination landscape. Our results hint at the primary influence of mechanistic features of meiotic pairing and synapsis rather than (epi)genomic features and..., , , # Recombination data for the repeat-based holocentromere-harbouring genome of *R. breviuscula*

This dataset provides complete raw sequencing data that is required for recombination analysis of *R. breviuscula*. Please refer to the following paper for more details and cite it when you use this dataset for publications:

Castellani M, Zhang M, Thangavel G, Mata-Sucre Y, Lux T, A. Campoy J. A., Marek M, Huettel B, Sun H, Mayer K. F. X., Schneeberger K & Marques A. (2023). Meiotic recombination dynamics in plants with repeat-based holocentromeres sheds light on the primary drivers of crossover patterning.

## Dataset

* **Phased genome assemblies of two haplotypes of *R. breviuscula***
`rhyBreHap1.fasta.gz`
`rhyBreHap2.fasta.gz`
* **Annotation of phased genome assemblies**
`rhyBreHap1_functAnno.gff3.gz`
`rhyBreHap2_functAnno.gff3.gz`
* **Single-cell RNA sequences from pollen nuclei by 10X Genomics**
`a4984_merged_R1.fastq.gz`
`a4984_merged_R1.fastq.gz`
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