Analysis of transcriptome post hypoxia and TGF-β treatment in breast cancer In order to explore the role of TGF-β signaling in mediating the alternative splicing program in breast cancer, we profiled
We used splicing-sensitive microarrays to detect differences in alternative splicing between two breast cancer cell lines MCF7 (estrogen receptor positive) and MDA-MB-231 (estrogen receptor negative),
In this study, combining 1) RNA-Sequencing on MCF-7 cell line, 2) a new computational approach to identify splice isoforms and 3) an extensive experimental validation (in vitro - on MCF-10, MCF-7 and