spatiotemporal single-cell transcriptomics in mouse and human to investigate TAM evolution during GBM progression. spatiotemporal single-cell transcriptomics in mouse and human to investigate TAM evolution during GBM progression

Glioblastoma (GBM) is the most common and malignant primary brain tumor. Although immunotherapy has shown promise in certain cancer types, it has not been effective against GBM, largely due to its highly immunosuppressive tumor microenvironment (TMEs), which is rich in tumor-associated macrophages/microglia (TAMs). TAMs in late-stage GBM contribute to T-cell exhaustion and worsen prognosis, but the role of TAMs in earlier stages of tumor development is unclear. By employing genetically engineered mouse models and human samples, we used spatiotemporal single-cell transcriptomics to investigate TAM evolution during GBM progression. Overall design: 1,Single immune cell (CD45+) sequencing of spatial sampling (paired Periphery, Border and Core) in 3 implanted QPP#7 mouse GBMs, and in 3 human GBM masses (Emory Cohort). 2,Single immune cell sequencing of Mg-TAMs and Mo-TAMs UPDATE: [Apr-17-2026] The processed data files for Samples GSM7714298, GSM7714300, GSM7714301, which were originally mislabeled and incorrectly associated, have been corrected. GSM7714298: Correct files were previously mislabeled as files for GSM7714300. GSM7714300: Correct files were previously mislabeled as files for GSM7714301. GSM7714301: Correct files were previously mislabeled as files for GSM7714298.