Flow cytometry phenotyping of non-sorted, sorted CD1a+, and sorted CD1a−negative cells from 3 control donors.

Cells were sorted at day 3 of monocyte differentiation, put back in culture for 4 additional days (iDC), and matured for 2 days with 10 ng/ml LPS (mDC).Minimum and maximum fluorescence values (number of sites per cell) of each group in each cell type were determined and results were scored as follows:0 site per cell: 0; low to 5 000 sites: ±; 5 000 to 10 000 sites: +; 10 000 to 100 000 sites: ++; >100 000 sites: +++.