Effect of loss of ciliogenesis on myoblast quiescence. Effect of loss of ciliogenesis on myoblast quiescence

Quiescence is an actively maintained state of the cell cycle;aberrations in which can result in severe consequencces for development and tissue homeostasis. Primary cilia are signaling centres derived from the centrosome which are associated with quiescence.Using a mouse skeletal myoblast cell culture system that can be induced to exit the cell cycle reversibly into quiescence or irreversibly into differentiation, we studied the effect of loss of ciliogenesis on quiescence. Overall design: We performed microarray analysisis to study changes in the global transcriptome of IFT88KD myoblasts in different cell cycle states. RNA was harvested from C2C12 myoblasts that underwent siRNA treatment using either IFT88 or negative control siRNA at four different cell cycle states- Proliferating (MB), Quiescent (G0), Reentry into cell cycle for 2 hours (R2), and 48 hours into terminal differentiation (D48). RNA was converted to cDNA, labelled and used to probe Affymetrix mouse 420A 2.0 arrays.