Ensifer Select and Resequence Experiments

Select and resequence experiments are a form of experimental evolution that involves creating an initial synthetic community from the mixture of multiple strains, allowing the community to evolve in a particular environment, and collecting the final community. By sequencing the initial and final communities, the effects of selection on strain and allele frequencies can be observed. These experiments used synthetic communities composed of Ensifer rhizobia (soil bacteria that fix nitrogen in association with legumes) evolved in various environments, including soil, plant hosts, and liquid culture.