Human motor neurons are rare in the spinal cord and can be transcriptomically divided into known subtypes divided into known subtypes.

Motor neurons are a rare neuronal subtype in the adult spinal cord. We performed single-nucleus RNA sequencing on nuclei extracted from adult human spinal cord and describe the transcriptional heterogeneity. Nuclei from the cervical spinal cord of a neurologically healthy control patient were isolated, homogenized, and stained with antibodies against neuronal marker NeuN. Nuclei were sorted by FANS and transcriptomes captured using 10x V3.1 3' kits.