2B_Vulva bursting quantification in heterochronic mutants

Quantification of vulva bursting of adult animals of indicated genotypes. Animals were grown at 25°C on NG2% OP50 plates and examined at 48 h of development. N = 3 technical replicates. Condition Strain code Genotype WT N2 WT let-7(-) HW2569 let-7(xe150) X; xeEx365[let-7p::let-7::SL1_operon::gfp, unc-119 (+); rab-3p::mCherry; myo-2p::mCherry; myo-3p::mCherry] lin-28(0) HW2705 lin-28(xe192) I lin-28(0); let-7(-) HW2792 lin-28(xe192) I; let-7(xe150) X lin-28(0); lin-46(0) HW3336 lin-28(xe192) I; lin-46(ma385) V lin-46(0) HW3506 lin-46(ma385) V lin-28(0); lin-46(0); let-7(-) HW3677 lin-28(xe192) I; lin-46(ma385) V; let-7(xe150) X; xeEx365[let-7p::let-7::SL1_operon::gfp, unc-119 (+); rab-3p::mCherry; myo-2p::mCherry; myo-3p::mCherry] lin-46(0); let-7(-) HW3678 lin-46(ma385) V; let-7(xe150) X; xeEx365[let-7p::let-7::SL1_operon::gfp, unc-119 (+); rab-3p::mCherry; myo-2p::mCherry; myo-3p::mCherry]