Diversity of post-translational modifications and signaling networks revealed by single cell and organelle mass spectrometry

Using an optimized data-independent acquisition approach on trapped ion mobility spectrometry, we measured proteins, post-translational modifications and variant peptides in single cells and single nuclei, which provided insights into heterogeneity of cell-state and signaling dependencies at the single cell level and the molecular impact of an epigenetic drug at the level of a single organelle.