Studying the quenching resulted from the formation of association complex between olsalazine or sulfasalazine with acriflavine - calibration curves sheets

We report the detection and quantification of important ulcerative colitis
drugs olsalazine (OLS) and sulfasalazine (SUL) by the Spectrofluorometric
method. The proposed method was optimized and validated by using the
quenching effect on the acriflavine fluorescence. The method was applied
on the detection and quantification of OLS and SUL under optimized
conditions showing the calibration curves were linear (Range:
1.0–10.0 µg/mL), with correlation coefficients R2 of 0.9999 for both
drugs. The limits of detection (LOD) and quantification (LOQ) were 53
ng/mL & 104 ng/mL for the OLS and 160 ng/mL & 315 ng/mL
for the SUL. This method permitted the analysis of OLS and SUL in their
pure and pharmaceutical forms. The proposed spectrofluorimetric method was
also evaluated against “green” criteria and all the experimental results
make it an eco-friendly and safe method for the detection of OLS and SUL.