The effect of ADAR1 depletion on mitochondrial proteins

In this study, we found that ADAR1 reduces the levels of double-stranded RNAs (dsRNAs) derived from both nuclear and mitochondrial transcripts. To elucidate how ADAR1 controls mitochondrial dsRNA (mt-dsRNA) accumulation, we performed quantitative mass spectrometry on mitochondrial protein fractions from wild-type and ADAR1-deficient cells, aiming to identify mitochondrial protein mediators involved in ADAR1-dependent dsRNA regulation.