High‑fidelity genome assembly of wheat introgression line Zahir‑1644

This dataset provides a high‑fidelity genome assembly of the wheat introgression line Zahir‑1644, generated using PacBio Sequel II Hi‑Fi reads and assembled with hifiasm (v0.19). The assembly, which has a contig N50 of 21 Mb, was quality‐assessed using QUAST (v5.2) and BUSCO (v5.1) against the ‘poales’ lineage, demonstrating high completeness and contiguity. These data support studies on wheat disease resistance mechanisms and genetic improvement. Included files comprise the raw sequencing reads, the assembled genome in FASTA format, and associated assembly metrics., Genomic DNA was extracted from the leaf tissues of Zahir-1644 introgressed line and sequenced through PacBio Hi-Fi sequencing platform. The raw reads were processed and then assembled using Hifiasm genome assembler., , # High‑fidelity genome assembly of wheat introgression line Zahir‑1644

[https://doi.org/10.1101/2024.08.30.610287](https://doi.org/10.1101/2024.08.30.610287)

Submitter Contact Information

Name: Karthick Gajendiran

Institution: King Abdullah University of Science and Technology, Thuwal, Saudi Arabia

Email: [[e-mail]](mailto:[e-mail])

Alternate Contact Information

Name: Brande Wulff

Institution: King Abdullah University of Science and Technology, Thuwal, Saudi Arabia

Email: [[e-mail]](mailto:[e-mail])

**Dataset Overview**

This dataset comprises the high‑fidelity genome assembly of the wheat introgression line Zahir‑1644, as described in the preprint \"A wheat tandem kinase sensor activates an NLR helper to trigger immunity\". The genome assembly is intended to support research in wheat genetics, particularly studies on disease resistance mechanisms.

**Key highlights include:**

Sequencing Platform:...,