Effect of stable and transient CB-D28k transfection on WNV titers.

*P≤0.05 t test.aTransfected with RSV-CB-Neo vector.bSerial dilutions of cell culture supernatant were added to indicator Vero 76 cells. Six days later cytopathic effect (CPE) was used to identify the end-point of infection [49], and 50% cell culture infectious doses (CCID50) per milliliter of cell culture supernatant.cTransfected with RSV-Neo control vector.dPercentage reduction of mean WNV titers from CB-D28k transfected cells compared to control transfected cells.eExperiments 1 and 2 consisted of stably transfected NSC34 cells with one cycle of cloning. Experiments 3 consisted of stably transfected NSC34 cells with three cycles of cloning. Experiments 4 and 5 consisted of transiently transfected NSC34 and Vero cells, respectively.fNumber of replicates.gTime of harvest after viral challenge.Effect of stable and transient CB-D28k transfection on WNV titers.