Establishment of an intradermal ear injection model of IL-17A and IL-36? as a tool to investigate the psoriatic cytokine network

The aim of this study was to establish an intradermal ear injection model to evaluate the effects of IL-36? and IL-17A in psoriasis.The cytokines IL-36? and IL-17A were injected intradermally alone or in combination into the ear pinnae of C57BL/6 mice. Ear thickness measurement was performed daily. After 4 days mice were euthanized and a gene expression profiling was conducted. Overall design: 12 samples were analyzed: Comparison between a PBS control group and groups that were treated with either IL-17A, IL-36? or the combination of both (3 samples of each group were analyzed).