Table S1 - Multiplex Detection of Plant Pathogens Using a Microsphere Immunoassay Technology

Plant pathogen detection in real infected samples. (A) Acicidovorax avenae subsp. citrulli (Aac) infected in watermelon (Citrullus lanatus), (B) chili vein-banding mottle virus (CVbMV) infected in Datura metel, (C) watermelon silver mottle virus (WSMoV) and (D) melon yellow spot virus (MYSV) infected in watermelon were diluted by six different dilution factors (DF) (1, 10, 50, 100, 500 and 1000-fold) and tested using three different assay formats (gold standard method, sandwich ELISA and microsphere immunoassay (MIA)). The gold standard method for the Aac detection was a sandwich ELISA where 11E5 is a capture antibody and MPC is a secondary antibody. The gold standard method for the CVbMV, WSMoV and MYSV detection is a plate-trapped antigen (PTA) ELISA with designated antibodies. MIA is a microsphere immunoassay. The signals obtained from the pathogen detection were normalized to the signal obtained from the detection in watermelon or Datura metel. The value from normalization was considered as a positive result (+) when it was above at least twice of background interpreting.
(DOCX)