Identification of long-lived proteins retained in cells undergoing repeated asymmetric divisions

Data from ProteomeXchange, PXD ID: PXD001251. File: ZN100309_092809_ZN_12.mzml. From ProteomeXchange: {{i}} To identify long-lived proteins that are retained in yeast mother cells, we combined a method developed in our lab with a pulse-chase protocol using total proteome analysis. Cells were initially grown in medium with stable heavy-isotope amino acids to label proteins. The cells were then switched into media containing light-isotope amino acids and cultured for an additional ~18 cell divisions with the MEP engaged. Finally, we determined whether any of the original heavy-labeled proteins were still present in the aged mother cells {{/i}}