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ALDH<sup>+</sup> and ALDH<sup>−</sup> subpopulations of breast cancer cells were separated by flow cytometer and cultured in stem cell medium as described in Materials and Methods.

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The numbers of tumosphere generated per 250, 500 or 1000 cells were counted two weeks later. (*p<0.05 vs ALDH−).

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2013-12-23
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