Dataset for embeRNA manuscript

This Figshare deposit includes datasets used to evaluate and apply embeRNA: Novel Microbes test set. A benchmark of 5,542 16S rRNA sequences whose maximum similarity to any 16S rRNA sequence in the embeRNA reference/training set is <97%. Metadata for these sequences are provided in: 5542_novel_16srRNA_fromNewAssemblies_information_lt97MaxSimiFromReferences_forFigShare.tsvSoil microbiome application (Fusion function predictions). We trained embeRNA models to predict Fusion functions and applied the V6–V8 embeRNA-Fusion model to real-world 16S rRNA soil samples1 (SRA project PRJNA434066). Predicted functional abundance profiles were CLR-transformed and statistically compared across soil compartments (rhizosphere vs. bulk), while adjusting for management effects. Significant differentially abundant Fusion functions are provided in: collingwoodSoil_184_and_1495_fusionFunctions_sigHigherInRhizosphereOrBulk_adjustManagement.tsvAssembly accession ids for the Balanced set organisms. Reference: Yurgel, S. N. et al. Variation in Bacterial and Eukaryotic Communities Associated with Natural and Managed Wild Blueberry Habitats. Phytobiomes 1, 102–113 (2017).