Low input RNA transcriptomic analysis of spleen and lymph node Fibroblastic Reticular Cells (FRCs) in a murine model of Diffuse Large B-cell Lymphoma (DLBCL)

Purpose: analyze the transcriptomic changes occurring in spleen (SP) and lymph node (LN) stromal cells in a murine model of DLBCL. Purpose: analyze the transcriptomic changes occurring in FRCs in DLBCL mRNA was extracted from flow sorted FRCs (gating on CD45-, CD31-, PDPN+ cells) isolated from spleen and lymph nodes of lymphoma I{mu}BCL6 and age-matched WT mice Human samples: mRNA was isolated from healthy LN FRCs (p3) left untreated or conditioned with human DLBCL cells (cell lines or primary tumor cells) for 48 hours using transwell (pore size 0.4µm). Primary FRCs directly isolated from DLBCL biopsies were also analyzed (p1)