Cyclic GMP-AMP synthase and interferon activation diminish MEF2C-mediated cognitive resilience III

To induce chronic IFN-I activation, we injected wildtype and Ifnar1–/– mice with five treatments of DMXAA, a STING agonist, followed by snRNA-seq analysis of hippocampal tissues. In wildtype mice, we showed that microglia had the strongest IFN response to STING activation of all cell types. Ablation of interferon alpha and beta receptor 1 (IFNAR1) abolished microglial IFN-I response and suppression of neuronal MEF2C transcriptional network Droplet-based single nuclear RNA sequencing of mouse hippocampus of WT and Ifnar1 KO mice with or without DMXAA treatment.