Next Generation Sequencing Facilitates Quantitative Analysis of transcriptomes in Human PBMC-derived MΦs and TAMs

The goals of NGS-derived macrophage transcriptome profiling (RNA-seq) is to identify the differences of signaling pathway between normal MΦs and tumor-associated macrophages (TAMs). Human PBMC-derived MΦs were generated in vitro, and TAMs were generated by using coculture with human myeloma cell lines U266 or ARP1 for 72 hours. Total RNA was extracted using RNeasy Mini Kit (Qiagen). 5-10 µg RNA samples were sent to BGI genomics for RNA-seq followed by data analysis. Examination of gene-profiling data in human PBMC-derived MΦs and TAMs.