Microsatellite data of the paper "A putatively new ant species from the Cataglyphis cursor group displays low levels of polyandry with standard sexual reproduction"

Fifty colonies of the ant Cataglyphis cursor were sampled at their nest entrance in two localities separated by 79km in the plain of Avila, west of Madrid, in July 2015 for Salobralejo (27 colonies) and in April 2014 for Castrillo de Guareña (23 colonies). Only workers near the nest entrance were collected. In Salobralejo, we also collected nine gynes at the nest entrance in three colonies. After collection, the individuals were preserved in 95% Ethanol (with 5% Tris-EDTA). From the 50 colonies sampled, we genotyped a single worker per colony for 37 colonies (Table S1). For the other 13 colonies, 114 workers were collected and genotyped to assess the colony genetic structure (86 workers from nine colonies in Salobralejo and 28 workers from four colonies in Castrillo). We also genotyped the nine gynes found in three colonies in Salobralejo. A total of 160 individuals were screened for thirteen microsatellite loci used by Eyer et al. (2023) and two supplementary loci (L76 and L3653) were amplified. The L26 locus was excluded because of amplification failures in nine individuals (18% of samples) and a highly significant Hardy-Weinberg disequilibrium (P < 00001 for both sites). 

The file contain the locality (Salobralejo or Castrillo), the colony number and individual identification number (code), the caste (worker or gyne). For each locus, the two alleles are provided and characterised by their size.