Whole genome sequencing, Amblyseius swirskii pooled population, ONT MinION -- ONT data I2 run 1 part I fast5

ReadMe: ONT data I2 runKim Ferguson, [e-mail] or [e-mail] University and Research
This ReadMe file is for all files associated with this project, and is present in each data package.
There are five .tar file sets in total available on figshare:> 20171130_1637_FAH35464_I2_run 1 part I fast5.tar> 20171130_1637_FAH35464_I2_run 1 part II fast5.tar> 20171201_0934_FAH35464_I2_run 1 restart 1 fast5.tar> 20171201_0954_FAH35464_I2_run 1 restart 2 nobasecalling fast5.tar> ONT data I2 run fastq.tar
Read creation dates: 30-11-2017 and 01-12-2017Albacore run date: 04-12-2017
Organism: Amblyseius swirskiiPopulation: Koppert biocontrol population, inbred for 10 generationsCollected: >1000 individuals for DNA extraction, males, females, juveniles, placed in 96% EtOHContamination: Collected with food source, pollen from Typha latifolia, and likely was part of DNA extractionExtraction protocol: QIAGEN MagAttract HMW DNA Kit (QIAGEN) according to the manual, while the final DNA product was eluted with nuclease free waterSequenced using ONT MinION sequencing technology
MinION model: MinION Mk1BFlowcell: FAH35464 FLO-MIN107 R9.5Ligation kit: Ligation Sequencing Kit version 108 (SQK-LSK108) (no genomic shearing)MinKNOW: version 1.10.16Reads that were basecalled in the cloud: > /20171130_1635_FAH35464_I2_run 1 mux> /20171130_1635_FAH35464_I2_run 1 full> /20171130_1635_FAH35464_I2_run 1 restart 1 mux> /20171130_1635_FAH35464_I2_run 1 restart 1 fullReads that were basecalled using Albacore (version 2.1.3):> /20171201_0952_FAH35464_I2_run 1 restart 2 nobasecalling mux> /20171201_0954_FAH35464_I2_run 1 restart 2 nobasecalling fullAlbacore results:> /basecalled_reads 20171201_0952_FAH35464_I2_run 1 restart 3 nobasecalling mux> /basecalled_reads 20171201_0954_FAH35464_I2_run 1 restart 3 nobasecalling full
Data further used in Paspati et al, in prep
Fun facts:
Files have been separated into fast5 and fastq files, with additional splitting into parts I and II for "/20171130_1635_FAH35464_I2_run 1 full" due to size. Each fast5 folder has been uploaded separateley
fast5 folders are often separated into Fail, Pass, and Skip, this is related to the cloud basecalling by the MinKNOW program, based on initial and ongoing quality assessments.
fastq folders and files would be considered raw and uncorrected, and were either basecalled in th cloud or using Albacore (see below). All fastq folders have been compressed and uploaded together.
There are two restarts in this sequencing run, due to MinKNOW crashing, either after a few hours or overnight. After the second crash, we decided to switch to no basecalling in hope that this would solve the issues.