Major group-B enterovirus populations deleted in the noncoding 5' region of genomic RNA modulate activation of the type I interferon pathway in cardiomyocytes and induce myocarditis

Major 5'-terminally deleted (5’TD) RNA forms of group-B coxsackievirus (CVB-5’TD) has been associated with myocarditis in both mice and humans. Although it is known that interferon-β (IFN-β) signaling is critical for an efficient innate immune response against CVB-induced myocarditis, the link between CVB-5’TD RNA forms and type I IFN signaling in cardiomyocytes remains to be explored. In a mouse model of CVB3/28-induced myocarditis, major early-emerging forms of CVB-5’TD RNA have been characterized as replicative viral populations that impair IFN-β production in the heart. Synthetic CVB3/28 RNA forms mimicking each of these major 5'TD virus populations were transfected in mice and have been shown to modulate innate immune responses in the heart and to induce myocarditis in mice. Remarkably, transfection of synthetic viral RNA with deletions in the secondary structures of the 5'-terminal CVB3 RNA domain I, modifying stem-loops “b”, “c” or “d”, were found to impair IFN-β production in hu..., This dataset includes the data results from: (i)In vivo, using DBA/2J mice for infection with Coxsackievirus B3 and viral RNA transfection and (ii) In vitro analysis, using human cardiomyocytes cells. The dataset includes the data of: RT-qPCR, ELISA, histological scorring, plaque forming units count, and quantification of 5'ends viral RNA (by microelectrophoresis with 2100 Bioanalyzer Instrument). , , # Data from: Major group-B enterovirus populations deleted in the noncoding 5' region of genomic RNA modulate activation of the type I interferon pathway in cardiomyocytes and induce myocarditis

[https://doi.org/10.5061/dryad.n2z34tn48](https://doi.org/10.5061/dryad.n2z34tn48)

This dataset includes the data results from: (i)*In vivo,* using DBA/2J mice for infection with Coxsackievirus B3 and viral RNA transfection, and (ii) *In vitro* analysis, using human cardiomyocytes cells. The dataset includes the data results obtained by : RT-qPCR, ELISA, histological scoring, plaque forming units count, and quantification of 5'non-coding Coxsackievirus B3 RNA (by microelectrophoresis with 2100 Bioanalyzer Instrument). 

## Description of the data and file structure

Our data raw are structured as follows:

**Figure 1_Data.xlsx:** Data representing the results summarized in Figure 1 (see Supplemental information figures): these data describe the dynamics of 5’terminally deleted Coxsackievirus B...