Puffy Skin Disease is an emerging transmissible condition in rainbow trout Oncorhynchus mykiss Walbaum. Oncorhynchus mykiss

The aim of this study was to test the hypothesis that Puffy Skin Disease (PSD) has an infectious aetiology, by undertaking a cohabitation experiment whereby naive triploid and diploid rainbow trout were cohabited with PSD-affected fish collected from the field. In addition, extensive additional investigations, including analysis of high-throughput sequencing datasets generated from skin samples of PSD-affected fish were undertaken in an attempt to identify potential infectious agents that may be responsible for PSD.