Sequences of Macrobrachium and outgroup species used this study.

Sample localities, locality abbreviations (Abbrev.), catalogue numbers, and GenBank accession codes are provided. The codes of sequences that were retrieved from GenBank are bold type.Museum/collection abbreviations: CCDB: Crustacean Collection of the Department of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo; MZUSP: Museu de Zoologia da Universidade de São Paulo; CNCR: Coleción Nacional de Crustaceos de la Universidad Nacional Autónoma de México; IVIC: Instituto Venezolano de Investigaciones Científicas; UCR: Museo de Zoología de la Universidad de Costa Rica; RMNHD: National Museum of Natural History, Leiden, The Netherlands.