Confirmation of plant viral proteins and identification of specific viral strains by nanoLC-ESI-Q-TOF using single-leaf-tissue samples

An efficient protein extraction protocol without any precipitation steps was applied to plant samples from ten economically important plant hosts. Viral proteins from fourteen important viruses (WDV, BYDV/BYDV-PAV, BMV, TAV, CaMV, TMV, TVCV, PPV, TuMV, BCMV, SrMV, SCMV, TRSV, BBWV-2) from seven different families (Geminiviridae, Luteoviridae, Bromoviridae, Caulimoviridae, Virgaviridae, Potyviridae, Secoviridae) were detected in infected samples. Protein database of host proteins and potential pathogen proteins was assembled separately for each host and based on existing online plant virus pathogen lists. For PPV, WDV, BYDV a succesfull discrimination to virus strains (as demonstrated for PPV, WDV) or distinct disease species (BYDV) was also demonstrated.