TurboID proximity labelling with protein TRIM52: Identification of biotinylated proteins through pull-down and label-free quantification

All constructs were expressed in RKO cells (human rectal carcinoma cell line). A fusion construct of TurboID (mutated BirA* from E. Coli) and green fluorescent protein (GFP) or human TRIM52. Cells were stimulated with Epoxomicin (proteasome inhibitor) or left untreated. Biotinylated proteins were extracted from the cell lysate through pull-down and quantified using label-free MS to identify the proteins in close proximity to TRIM52 using GFP as a specificity control.