Gene expression changes after Ctcfl overexpression in NIH3T3 cells.. Mus musculus

The two paralogous zinc finger factors CTCF and CTCFL differ in expression such that CTCF is ubiquitously expressed, whereas CTCFL is found during spermatogenesis and in some cancer types. Both factors share the highly conserved DNA binding domain and are bound to DNA sequences with an identical consensus. Here we analyzed the differential gene expression effect mediated by expression of Ctcfl in undifferentiated and differentiated NIH3T3 cell. Overall design: NIH3T3 cells stably transfected with inducible Ctcfl expression vectors were used for RNA extraction and hybridization on Affymetrix microarrays. We analyzed gene expression in the presence/absence of Ctcfl.