5-methylcytosine (5mC) is the most important DNA modification in mammalian genomes as a lineage-defining mark dynamically altered in development and disease. The ideal method for 5mC localization woul

NIAID Data Ecosystem20

Whole human genome methylation analysis of the HEL cell line using nanopore sequencing

表观遗传学测序

白血病细胞甲基化分析

In this work, we describe the whole genome sequencing and 5’-methylcytosine (5’-mC) calling of the human erythroleukemia (HEL) cell line. Genomic DNA libraries were prepared using a rapid library prep

NIAID Data Ecosystem100

DNA methylation drift as a marker of lifespan in mammals [human]

表观遗传学测序

衰老生物标志物

We analyzed levels of 5-methyl cytosine CCCGGG target sites by sequential restriction digest by SmaI and XmaI enzymes, ligating Illumina adaptors to the restriction fragments and reading methylation-s

NIAID Data Ecosystem30

small RNA deep-sequencing of mature sperm, testis and uterus in mice

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