Comparison of the current density as a function of time for the WT CLC-1 and the A531V mutant.

All data were obtained from whole-cell patch-clamp recordings in tsA201 cells. (A) Recording of WT CLC-1 4–7 hrs after transfection. (B) Recordings of the A531V mutant at the indicated time periods after transfection. (C) Averaged instantaneous current-voltage (I-V) curves of the A531V mutant during the four time periods indicated in B. Current amplitude is shown in the form of current density (Id; whole cell current/cell capacitance). Colors of the symbol represent: Black, 4–7 hrs (n = 14); Blue, 8–11 hrs (n = 7); Green, 24–27 hrs (n = 5); Red, 48–51 hrs (n = 4). (D) Current density of WT and A531V as a function of time after transfection. The instantaneous current at Vm = −120 mV was used for the calculation. The dotted arrow for WT (black color) represents the fact that the cells were un-clampable at 24 hrs after transfection. (E) Steady-state Po–V curves of the WT CLC-1 and the A531V mutant.