Genome wide profiling of chromatin accessibilities in murine mononuclear phagocyte lineage progenitor cells

To understand the mechanism underlying monocyte and dendritic cell development through the regulation of Irf8 expression by the 56 kb downstream (+56 kb) Irf8 enhancer, we analyzed chromatin accessibility of bone marrow cells from wild-type and Irf8-/- mice. Combined with the epigenetic profiling by H3K27 acetylation ChIP-seq, the +56 kb enhancer opens at LMPP stage, and gets activated at its highest level in mononuclear phagocyte lineage progenitor cells. Purified transposed DNA from murine long-term hematopoietic stem cells (LT-HSC), lymphoid-primed multipotent progenitors (LMPP), monocyte-dendritic cell progenitors (MDP), common dendritic cell progenitors (CDP) in wild-type (WT) mice was subjected to ATAC-seq (biological duplicates for each cell type).