Gene expression analysis of jam3b mutant embryos

Junctional adhesion molecule 3 (Jam3) is a cell adhesion molecule that is involved in cell-cell interaction, cell migration, and cell polarization in many tissue including the epithelium, endothelium, and hematopoietic cells. Although Jam3 is functionally relevant for both hematopoietic and vascular endothelial cells, the role of Jam3 in hematopoietic or vascular development is still unknown. In this study, a zebrafish jam3b mutant line, jam3b-sa37, was used for the cap analysis gene expression (CAGE) sequencing to identify genes that are involved in hematopoietic and/or vascular development in the zebrafish embryo. Whole embryos mRNA profiles of wild type or jam3b-sa37 embryos at 12 or 16 hours post-fertilization were generated by CAGE sequencing using Illumina NextSeq500.