AMPK mutant dauer transcriptome

C. elegans lacking AMPK do not exhibit reproductive defects during normal growth, but when passaged through dauer, exhibit germline hyperplasia and premature spermatogenesis, followed by complete sterility post-dauer (Narbonne and Roy, Development, 2006). Loss of RNAi pathway components suppresses several of these phenotypes (Kadekar and Roy, PLoS Biol, 2019). We sought to carry out a transcriptomic analysis of control and AMPK mutant animals in the dauer and post-dauer stage to understand what gene expression changes occur due to the absence of AMPK, and how RNAi pathway components may be affected. The daf-2 mutation was used to induce dauer by a temperature shift to 25 °C. RNA-Seq of dauer (96 hours post-hatching at restricted temperature) and post-dauer (96 hours after shift to permissive temperature) in the daf-2 and daf-2; aak-1; aak-2 (aak(0)) genetic background. Standard Trizol RNA extraction of collected samples.