btuE expression results in decreased intracellular ROS.
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Cytoplasmic ROS content was assessed by measuring the activation of 2′,7′-dihydrodichlorofluorescein diacetate in wild type, pBAD/btuE, ΔbtuE and ΔbtuE pBAD/btuE cells as described in Methods. Cells were induced with 0.2% arabinose and exposed to K2TeO3 (0.5 µg ml−1), paraquat (50 µg ml−1) or K2CrO4 (1 mM) for 15 min at 37°C. Fluorescence (AU, arbitrary units) was determined and normalized per mg of protein. Values represent the mean of three independent trials ± SD.
创建时间：
2015-12-02
