Flt3L alters CD11c expression on splenic DC populations and increases the gene expression of the DC associated genes Flt3 and BLIMP-1.

A) Representative histograms of CD11c expression on splenic DC populations (Dashed line = isotype, tinted grey = sham-treated, black = Flt3L-treated). B) Relative increase (%) in the intensity of CD11c expression (measure by gMFI) on splenic DC populations after Flt3L-treatment compared to sham treated mice C) Gene expression of Flt3 and Blimp-1 in the spleen at day 28. D) Representative FACS plot of Flt3 expression on splenic CD11cHI lymphocytes. E) Correlation between Blimp-1 and Flt3 gene expression in the spleen. The relative increase of CD11c expression was calculated as the percental change compared to sham-treated mice within the same experiment. Data are presented as mean ± SEM and statistical significance was assessed using unpaired t-test. *PPP